COMPUTER-ASSISTED AND CONVENTIONAL SPERM MOTILITY ANALYSIS OF BOER AND ALPINE GOATS SEMEN IN THE AUTUMN AND SPRING.

T. V CAVALCANTE, C. R. ESPER, H. C. AZEVEDO, M. F. CORDEIRO

Abstract


One of the limiting factors in the caprine reproduction field is semen production and quality. To evaluate semen, the analysis of sperm motility is the most common procedure used in order to diagnose male?s fertility. The aim of this work was to evaluate the effect of breed, buck, analysis method, and season (autumn and spring) on sperm motility. A total of 71 ejaculates were collected in autumn and 64 in springtime from Boer (n=5) and Alpina (n=4) bucks using an artificial vagina. After collection, semen samples were diluted in PBS(phosphate saline buffer), in order to reach a concentration of 50 million cells/mL for computer analysis. A second sample was diluted in 0.9% saline solution for conventional analysis. Data concerning different breeds, bucks, methods and seasons were analyzed by ANOVA and Tukey test. No statistical differences were observed in relation to breeds and seasons. However, within breeds, there was a significant statistical difference (p>0.01) for sperm motility in relation to the method used for evaluation. In conclusion, conventional and computer-based sperm motility in Boer and Alpina males did not show changes regarding season, though there was an individual variation among males, which was breed independent.
KEY-WORDS: Caprine. Semen. Sperm motility. CASA. Seasonality.



DOI: http://dx.doi.org/10.15361/2175-0106.2005v21n4p203-208